Method of Analysis of Herbs - II

Chinese name

Herb name

Method of Analysis

Key ingredients

Assay

肉苁蓉

Roucongrong

Cistanches   Herba

Aglient   Zorbax Eclipse Plus C18,150mm   x 4.6mm ,5μm,5μl,   λ:330nm,30.0℃,   1ml/min;Mobile phase:A:0.1%Formic acid;B:Methanol;B:   0-17min,26.5%;17-20min,26.5-32%;20-27min 32%;27-28min 32-70%;28-30min   70%;30-31min 70-26.5%

Echinacoside

Verbascose

0.75%

0.27%

肉豆蔻

Roudoukou

Myristicae   Semen

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:274nm,30.0℃, 1ml/min;Mobile   phase: Methanol:Water=75:25

Dehydrodiisoeugenol

0.70%

肉桂

Rougui

Cinnamomi   Cortex

Aglient   Eclipse Plus C18,250mm x 4.6mm ,5μm,20μl, λ:290nm,26.8℃, 1ml/min;Mobile   phase: Acetonitrile:Water=32:68

Cinnamic   aldehyde

0.22%

朱砂根

Zhushagen

Ardisiae   Crenatae Radix

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:275nm,28.0℃, 1ml/min;Mobile   phase:Methanol:0.1%Phosphoric acid =25:75

Bergenin

0.82%

延胡索

Yanhusuo

Corydalis   Rhizoma

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:280nm,28.0℃, 1ml/min;Mobile   phase:Methanol:0.1%Phosphoric acid (PH=6)=40:60

Tetrahydropalmatine

0.11%

伊贝母

Yibeimu

Fritillariae   Pallidiflorae Bulbus

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, 1ml/min;The   atomization temperature: 60.0℃;Mobile   phase:Acetonitrile:Wate:Diethylamine=55:45:0.03

Sipeimine   glycosides

Sipeimine

0.25%

0.022%

血竭

Xuejie

Draconis   Sanguis

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:440nm,40.0℃, 1ml/min;Mobile   phase:Acetonitrile:0.05mol/L NaH2PO4 =50:50

Dracorhodin

0.088%

合欢花

Hehuanhua

Albiziae   Flos

Aglient   Zorbax Extend C18,250mm   x 4.6mm ,5μm,20μl,   λ:256nm,25.0℃,   1ml/min;Mobile phase:   Acetonitrile:0.1%Phosphoric acid =22:78

Quercitrin

1.36%

决明子

Juemingzi

Cassiae   Semen

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:284nm,25.0℃, 1ml/min;Mobile phase: A:0.1%Phosphoric   acid,B:Acetonitrile;B:0-15min 40%,15-30min 40-90%,30-40min 90%,40-41min   90-40%,41-50min 40%

Chrysophanol

Orange   obtusin

1.13%

0.40%

灯盏细辛

Dengzhanxixin

Erigerontis   Herba

Aglient   Eclipse Plus C18,250mm x 4.6mm ,5μm,20μl, λ:335nm,28.0℃, 1ml/min;Mobile   phase: Methanol: 0.1%Phosphoric acid=40:60

Scutellarin

1.80%

安息香

Anxixiang

Benzoinum

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:228nm,25.0℃, 1ml/min;Mobile   phase: Methanol:Water:Acetic acid=47:53:0.2

Total   balsamic acid

(Benzoic   acid)

29.16%

防己

Fangji

Stephaniae   Tetrandrae Radix

Aglient   Eclipse Plus C18,250mm x 4.6mm ,5μm,20μl, λ:280nm,27.0℃, 1ml/min;Mobile   phase: Acetonitrile: Methanol:Water: Glacial acetic acid=40:30:30:1

Fangchinoline

(+)-Tetrandrine

0.53%

1.57%

防风

Fangfeng

Saposhnikoviae   Radix

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,40μl, λ:254nm,27.9℃, 1ml/min;Mobile   phase: Methanol:Water =40:60

prim-O-glucosylcimifugin,5'-O-glucopyranosyl-5-O-methylvisamminol

0.70%

红花

Honghua

Carthami   Flos

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:403nm,25.0℃, 1ml/min;Mobile   phase: Methanol:Acetonitrile: 0.7%Phosphoric acid =26:2:72

Hydroxysafflor   yellow A

0.83%

红景天

Hongjingtian

Rhodiolae   Crenulatae Radix et rhizome

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:275nm,26.2℃, 1ml/min;Mobile   phase: Methanol:Water=15:85

Salidroside

0.92%

远志

Yuanzhi

Polygalae   Radix

Aglient   Eclipse Plus C18,150mm x 4.6mm ,5μm;10μl, λ:210nm,25.0℃, 1ml/min;Mobile   phase: Methanol: 0.05%Phosphoric acid =65:35

Tenuifolin

2.73%

远志

Yuanzhi

Polygalae   Radix

Aglient   Extend-C18,250mm x 4.6mm ,5μm,10μl, λ:320nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile: 0.05%Phosphoric acid =15:85

Polygalaxanthone   III

3,6’－Disinapoylsucrose

0.15%

0.27%

赤芍

Chishao

Paeoniae   Radix Rubra

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:230nm,28.0℃, 1ml/min;Mobile   phase: Methanol: 0.05mol/L KH2PO4=32:68

Paeoniflorin

3.61%

芫花

Yuanhua

Genkwa   Flos

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:338nm,25.0℃, 1ml/min;Mobile   phase: Methanol:Water: Glacial acetic acid=65:35:0.8

Genkwanin

0.59%

芥子

Jiezi

Sinapis   Semen

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,10μl, λ:326nm,30.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.08mol/L KH2PO4

Sinapine   thiocyanate

0.63%

芦荟

Luhui

Aloe

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:355nm,28.0℃, 1ml/min;Mobile   phase: Acetonitrile:Water=25:75

Aloin

14.7%

苏合香

Suhexiang

Styrax

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:285nm,27.4℃, 1ml/min;Mobile   phase: Methanol: 1%Glacial acetic acid=50:50

Cinnamic   acid

0.13%

杜仲

Duzhong

Eucommiae   Cortex

Aglient   Eclipse Plus C18,150mm x 4.6mm ,5μm,20μl, λ:277nm,28.3℃, 1ml/min;Mobile   phase: Methanol:Water=25:75

Pinoresinol   ,Diglucoside

0.43%

杜仲叶

Duzhongye

Eucommiae   Folium

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:327nm,26.2℃, 1ml/min;Mobile   phase: Acetonitrile:Water=13:87

Chlorogenic   acid

1.42%

豆蔻

Doukou

Amomi   Fructus Rotundus

HP-Innowax,30m x 0.25mmx0.25μm20μl, Inlet:190℃,Detector:230℃,Column:50℃-80℃.   Detector:FID

Eucalyptol

0.036%

两头尖

Liangtoujian

Anemones   Raddeanae Rhizoma

Aglient   Eclipse Plus C18,150mm x 4.6mm ,5μm,20μl, λ:206nm,30.0℃, 1ml/min;Mobile   phase: A: Acetonitrile;B: 0.1%Phosphoric acid;A: 0-7min 47%,7-15min 47-55%

Raddeanin(Anemodeanin)A

0.13%

两面针

Liangmianzhen

Zanthoxyli   Radix

Aglient   Zorbax Eclipse Plus C18,150mm   x 4.6mm ,5μm,10μl,   λ:273nm,30.0℃,   1ml/min;Mobile phase: A:0.1% Formic acid- triethylamine(pH4.5),B:   Acetonitrile;B: 0-30min 20-50%;30-35min,50-100%

Nitidine   chloride

0.05%

连翘

Lianqiao

Forsythiae   Fructus

Aglient   Zorbax Eclipse plus C18,150mm   x 4.6mm ,5μm,20μl,   λ:277nm,25.0℃,   1ml/min;Mobile phase:   Acetonitrile:Water=25:75

Phillyrin

0.074%

连翘

Lianqiao

Forsythiae   Fructus

Aglient   Zorbax Eclipse plus C18,150mm   x 4.6mm ,5μm,20μl,   λ:330nm,25.0℃,   1ml/min;Mobile phase:   Acetonitrile:0.4%Acetic acid =15:85

Forsythiaside   A

0.25%

吴茱萸

Wuzhuyu

Euodiae   Fructus

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm;20μl, λ:225nm,30.0℃, 1ml/min,Mobile phase: Acetonitrile:Water:   Tetrahydrofuran: Acetic acid =41:59:1:0.2

Limonin

Evodiamine

Rutaecarpine

1.16%

1.12%

0.48%

牡丹皮

Mudanpi

Moutan   Cortex

Aglient   Eclipse plus C18,150mm x 4.6mm ,5μm,20μl, λ:274nm,26.9℃, 1ml/min;Mobile   phase: Methanol:Water=45:55

Paeonol

1.43%

何首乌

Heshouwu

Polygoni   Multiflori radix

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:320nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile:Water=25:75

2,3,5,4'-Tetrahydroxystilbene-2-O-b-D-glucopyranoside

3.70%

何首乌

Heshouwu

Polygoni   Multiflori radix

Aglient   Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, λ:354nm,25.0℃, 1ml/min;Mobile   phase: Methanol:0.1%Phosphoric acid =80:20

Emodin

Physcion

0.11%

0.15%

佛手

Foshou

Citri   Sarcodactylis Fructus

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:284nm,25.0℃, 1ml/min,Mobile phase: Methanol:Water: Acetic   acid =35:63:2

Hesperidin

0.060%

余甘子

Yuganzi

Fructus   Phyllanthi

Aglient   Zorbax SB-Aq C18,250mm x 4.6mm ,5μm,20μl, λ:273nm,30.0℃, 1ml/min,Mobile phase: Methanol:   0.2%Phosphoric acid =4:96

Gallic   acid

1.62%

辛夷

Xinyi

Magnoliae   Flos

Aglient   Eclipse XDB C18,250mm x 4.6mm ,5μm,20μl, λ:278nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile: Tetrahydrofuran:Water=35:1:64

Magnolin

1.71%

羌活

Qianghuo

Notopterygii   Rhizoma Et Radix

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:310nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile:Water=44:56

Notopterol

Isoimperatorin

0.65%

0.18%

沙苑子

Shayuanzi

Astrgali   Complanati Semen

Aglient   Zorbax Eclipse plus C18,150mm   x 4.6mm ,5μm,10μl,   λ:266nm,30.0℃,   1ml/min;Mobile phase: Acetonitrile:   0.1%Phosphoric acid =21:79

Complanatoside   A

0.21%

沙棘

Shaji

Hippophae   Fructus

Aglient   Eclipse plus C18,150mm x 4.6mm ,5μm,20μl, λ:370nm,25.0℃, 1ml/min;Mobile   phase: Methanol:0.4%Phosphoric acid =58:42

Isorhamnetin

0.11%

补骨脂

Buguzhi

Psoraleae   Fructus

Aglient   Zorbax SB C18,250mm x 4.6mm ,5μm,20μl, λ:246nm,27.0℃, 1ml/min,Mobile phase: Methanol:Water=55:45

Psoralen

Isopsoralen

0.20%

0.23%

陈皮

Chenpi

Citri   Reticulatae Pericarpium

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:283nm,28.0℃, 1ml/min;Mobile   phase: Methanol:Acetic acid:Water=35:4:61

Hesperidin

4.48%

忍冬藤

Rendongteng

Lonicerae   Japonicae Caulis

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,20μl, λ:327nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile: 0.4%Phosphoric acid =10:90

Chlorogenic   Acid

0.13%

忍冬藤

Rendongteng

Lonicerae   Japonicae Caulis

Aglient   Zorbax SB-Phenyl,150mm   x 4.6mm ,5μm,20μl,   λ:236nm,25.0℃,   1ml/min;Mobile phase: Acetonitrile:   0.4%Phosphoric acid =12:88

Loganin

0.14%

青风藤

Qingfengteng

Sinomenii   Caulis

Aglient   Extend C18,250mm x 4.6mm ,5μm,20μl, λ:262nm,28.0℃, 1ml/min;Mobile   phase: Methanol: Phosphate buffer =48:52

Sinomenine

1.04%

青皮

Qingpi

Citri   Reticulatae Pericarpium Viride

Aglient   Extend C18,150mm x 4.6mm ,5μm,20μl, λ:284nm,26.8℃, 1ml/min;Mobile   phase: Methanol: Water=25:75

Hesperidin

6.45%

青黛

Qingdai

Indigo   Naturalis

Aglient   Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, λ:606nm,25.0℃, 1ml/min;Mobile   phase: Methanol: Water=75:25

Indigo

14.26%

青黛

Qingdai

Indigo   Naturalis

Aglient   Eclipse plus C18,250mm x 4.6mm ,5μm,20μl, λ:292nm,25.0℃, 1ml/min;Mobile   phase: Methanol: Water=70:30

Indirubin

0.15%

苦玄参

Kuxuanshen

Picriae   Herba

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:264nm,35.0℃, 1ml/min;Mobile   phase: Acetonitrile:Water=35:65

Picfeltarraenin   I A

0.53%

苦地丁

Kudiding

Corydalis   Bungeanae Herba

Aglient   Zorbax SB-C18,250mm x 4.6mm ,5μm,10μl, λ:289nm,28.0℃, 1ml/min;Mobile   phase: Methanol:0.015mol/L Phosphate buffer =70:30

Corynoline

0.64%

苦杏仁

Kuxingren

Armeniacae   Semen Amarum

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:207nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.1%Phosphoric acid =8:92

Amygdalin

19.61%

苦参

Kushen

Sophorae   Flavescentis Radix

Aglient   Zorbax NH2, 250mm x 4.6mm ,5μm,20μl, λ:220nm,27.7℃, 1ml/min;Mobile   phase: Acetonitrile:Anhydrous ethanol=80:20

Matrine

Oxymatrine

0.83%

0.67%

苦楝皮

Kulianpi

Meliae   Cortex

Aglient   Zorbax SB-C18,150mm x 4.6mm ,5μm,20μl, λ:207nm,25.0℃, 1ml/min;Mobile   phase: Acetonitrile:0.01% Formic acid =31:69

Toosendanin

0.007%

板蓝根

Banlangen

Isatidis   Radix

Aglient   Extend C18, 250mm x 4.6mm ,5μm,20μl, λ:245nm,25.0℃, 1ml/min;Mobile   phase: Methanol:0.02%Phosphoric acid =7:93

Epigoitrin

0.020%

刺五加

Ciwujia

Acanthopancis   Senticosi radix et rhizoma seu caulis

Aglient   Eclipse plus C18,250mm x 4.6mm ,5μm,40μl, λ:265nm,27.7℃, 1ml/min;Mobile   phase: A:Acetonitrile, B:Water;A: 0-20min,10%-20%

Syringin

0.15%