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Method of Analysis for some natural ingredients

 

Below MOA is for reference only.

 

Product   name

CAS   number

HPLC   MOA

1-Deoxynojirimycin

19130-96-2

ELSD:Acetonitrile:Water=80:20

Allicin

539-86-6

HPLC-UV:Methanol:0.1%Formic   acid=75:25;λ:210nm

Alliin

556-27-4

RP-HPLC:Methanol:Water=30:70;λ:214nm

Aloeemodin

481-72-1

RP-HPLC:Methanol:Water:Phosphoric   acid=40:60:0.2;λ:360nm

Amygdalin

29883-15-6

RP-HPLC:Methanol:Water=20:80;λ:210nm

Andrographolide

5508-58-7

RP-HPLC:Methanol:Water=52:48;λ:225nm

Arbutin

497-76-7

RP-HPLC:Methanol:Water=20:80;λ:282nm

Artemether

71963-77-4

RP-HPLC:Methanol:Water=77:23;λ:343nm

Artemisinin

63968-64-9

RP-HPLC:Methanol:Water=72:28;λ:260nm

Artesunate

88495-63-0

RP-HPLC:Acetonitrile:Phosphate   buffer

Asiaticoside

16830-15-2

RP-HPLC:Acetonitrile:Water:2mmol/L   Beta cyclodextrin=24:76;λ:205nm

Baicalin

21967-41-9

RP-HPLC:Methanol:Water:Acetic   acid=35:61:4;λ:335nm

Bilobalide

33570-04-6

HPLC-ELSD:Methanol:Tetrahydrofuran:Water=25:10:65

Carnosic   acid

3650-9-7

RP-HPLC:Acetonitrile:0.5%Phosphoric   acid=75:25;λ:230nm

Catechin

154-23-4

HPLC-UV:KH2PO4(PH2.5):Methanol   Gradient elution;λ:280nm

Chlorogenic   acid

327-97-9

RP-HPLC:Acetonitrile:0.4%Phosphoric   acid=13:87;λ:327nm

Chrysin

480-40-0

HPLC:Methanol:0.15%Phosphoric   acid=64:36,λ:268nm

Curcumin

458-37-7

HPLC:Acetonitrile:4%Glacial   acetic acid=48:52,λ:430nm

Diacerein

13739-02-1

RP-HPLC:Acetonitrile:Water=75:25;λ:254nm

Dihydroartemisinin

71939-50-9

RP-HPLC:Acetonitrile:Methanol:0.02mol/L   Ammonium sulphate=50:10:40

Diosmin

520-27-4

RP-HPLC:Acetonitrile:Glacial   acetic acid:Methanol:Water=2:6:28:66,λ:275nm

EGCG

989-51-5

HPLC:Methanol:Water=90:10,λ:230nm

Eleutheroside   B

118-34-3

RP-HPLC:Methanol:Water=20:80;λ:265nm

Eleutheroside   E

39432-56-9

RP-HPLC:Methanol:0.1%Phosphoric   acid=32:68;λ:220nm

Ellagic   acid

476-66-4

RP-HPLC:Methanol:0.5%Glacial   acetic acid Gradient elution,λ:254nm

Ferulic   acid

1135-24-6

RP-HPLC:Methanol:Water(40:60);λ:320nm

Gallic   acid

149-91-7

RP-HPLC:Methanol:Water=40:60;λ:250nm

Genistein

446-72-0

RP-HPLC:Methanol:Water=60:40;λ:260nm

Ginkgolic   acid

20261-38-5

RP-HPLC:Methanol:1%Glacial   acetic acid=90:10;λ:310nm

Ginsenoside


RP-HPLC:Acetonitrile:Water   Gradient elution,λ:203nm

Hesperidin

520-26-3

HPLC:Methanol:Acetic   acid:Water=35:4:36,λ:283nm

Honokiol

35354-74-6

RP-HPLC:Methanol:Water=78:22;λ:294nm

Huperzine   A

102518-79-6

RP-HPLC:Methanol:Water=85:15;λ:231nm

Hydroxytyrosol

10597-60-1

RP-HPLC:Methanol:0.125%Glacial   acetic acid=10:90,λ:430nm

Icariin

489-32-7

RP-HPLC:Acetonitrile:0.2%   acetic acid Gradient elution;λ:270nm

Levodopa

59-92-7

RP-HPLC:0.05mol/L   KH2PO4:Methanol:Acetonitrile=70:25:5

Lovastatin

75330-75-5

RP-HPLC:Acetonitrile:Water=60:40;λ:239nm

Luteolin

491-70-3

RP-HPLC:Acetonitrile:0.3%Phosphoric   acid=30:70;λ:349nm

Lycopene

502-65-8

RP-HPLC:Acetonitrile:Tetrahydrofuran:Dichloromethane=85:3.4:1.6;λ:472nm

Magnolol

528-43-8

RP-HPLC:Methanol:Water=78:22;λ:294nm

Mogroside   V

88901-36-4

RP-HPLC:Acetonitrile:Water=23:77;λ:203nm

Naringin

10236-47-2

RP-HPLC:Methanol:Water:Acetic   acid=35:61:4;λ:283nm

Neohesperidin

13241-33-3

RP-HPLC:Acetonitrile:Water=20:80;λ:283nm

Neohesperidin   Dihydrochalcone

20702-77-6

RP-HPLC:Acetonitrile:0.2%Phosphoric   acid=25:75;λ:282nm

Notoginsenoside   R1

80418-24-2

RP-HPLC:Acetonitrile:Water   Gradient elution,λ:203nm

Nuciferine

475-83-2

RP-HPLC:Acetonitrile:Water:Triethylamine:Glacial   acetic acid=27:70.6:1.6:0.78;λ:270nm

Oleuropein

32619-42-4

RP-HPLC:Acetonitrile:0.2%Phosphoric   acid=25:75;λ:285nm

Osthole

484-12-8

RP-HPLC:Acetonitrile:Water=49:51,λ:330nm

Paeoniflorin

23180-57-6

RP-HPLC:Acetonitrile:0.1%Phosphoric   acid=14:86,λ:230nm

Polydatin

27208-80-6

RP-HPLC:Acetonitrile:Water=23:77;λ:306nm

Procyanidin

4852-22-6

RP-HPLC:Water:Methanol:Isopropyl   alcohol:Formic acid=73:13:6:8;λ:525nm

Punicalagin

65995-63-3

RP-HPLC:Methanol:Glacial   acetic acid=50:50;λ:280nm

Quercetin

6151-25-3

RP-HPLC:Methanol:0.5%Phosphoric   acid=47:53;λ:360nm

Resveratrol

501-36-0

RP-HPLC:Acetonitrile:1.0%Phosphoric   acid=30:70,λ:303nm

Rosavin

84954-92-7

RP-HPLC:Methanol:Water=40:60;λ:250nm

Rosmarinic   acid

20283-92-5

RP-HPLC:Acetonitrile:0.1%Phosphoric   acid=20:80;λ:342nm

Rutin

153-18-4

RP-HPLC:Acetonitrile:Methanol:0.4%Acetic   acid=16:8:76,λ:360nm

Salidroside

10338-51-9

HPLC-UV:Methanol:Water=15:85;λ:275nm

Shikimic   acid

138-59-0

RP-HPLC:Acetonitrile:0.2%Phosphoric   acid=90:10;λ:213nm

Silibinin

22888-70-6

RP-HPLC:Methanol:Water=70:30;λ:288nm

Stevioside

57817-89-7

RP-HPLC:Methanol:Water=68:32;λ:205nm

Ubidecarenone

303-98-0

RP-HPLC:Methanol:Anhydrous   ethanol=50:50;λ:275nm

Xanthophyll

127-40-2

RP-HPLC:Methanol:Acetonitrile:Ethyl   acetate:Water=30:29:40:1;λ:450nm

β-Carotene

7235-40-7

RP-HPLC:Methanol:Acetonitrile:Dichloromethane=60:30:10;λ:435nm